Western blot analysis from lysates of canine peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 5 µg/mL PMA and 500 ng/mL Ionomycin overnight. PVDF Membrane was probed with 1 µg/mL of Canine CCL4/MIP-1ß Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for CCL4/MIP-1ß at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions.